Flow Cytometry Analysis Protocol
Excellent flow cytometry analysis protocol from the Springer Lab. Please follow the link for the entire protocol.
Flow Cytometry Analysis
Purpose
Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based on the measurement of visible and fluorescent light emission. Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules.
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