Susan Forsburg’s method for Schizosaccharomyces pombe
Reagents and Protocol
1. Spin down 107 cells from an exponentially growing culture - 2000 rpm for 5 mins. Pour off supernatant.
For the rest of the protocol please click on the above link.
Excellent flow cytometry analysis protocol from the Springer Lab. Please follow the link for the entire protocol.
Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based on the measurement of visible and fluorescent light emission. Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules.