Peripheral Blood Cell Flow Cytometry Analysis
Thursday, March 25th, 2010FACS Analysis Using Peripheral Blood Cells
Click on above link for entire protocol.
1. Collect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix immediately to prevent clotting. Keep tubes on ice.
2. Remove RBCs from samples. This can be accomplished by several means. At The Jackson Laboratory, RBCs are lysed using either Gey’s solution or a buffered ammonium chloride (ACK) solution. (Alternatively, Becton-Dickinson sells a product called “FACS lysis buffer” that is used after the staining protocol to lyse RBCs and fix the cells.)