Flow Cytometry

Intracellular Immunofluorescent Staining for Flow Cytometry

Intracellular Immunofluorescent Staining for Flow Cytometric Analysis (FACS Analysis)

A modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular antigens at the single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens as in the surface antigen protocol, then fixed with paraformaldehyde to stabilize the cell membrane and permeabilized with the detergent saponin to allow anti-cytokine antibodies to stain intracellularly.